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Mercury in PDB 1aro: T7 Rna Polymerase Complexed with T7 Lysozyme

Enzymatic activity of T7 Rna Polymerase Complexed with T7 Lysozyme

All present enzymatic activity of T7 Rna Polymerase Complexed with T7 Lysozyme:
2.7.7.6; 3.5.1.28;

Protein crystallography data

The structure of T7 Rna Polymerase Complexed with T7 Lysozyme, PDB code: 1aro was solved by T.Steitz, D.Jeruzalmi, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 30.00 / 2.80
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 273.385, 95.612, 63.582, 90.00, 101.40, 90.00
R / Rfree (%) 26.2 / 30.9

Mercury Binding Sites:

The binding sites of Mercury atom in the T7 Rna Polymerase Complexed with T7 Lysozyme (pdb code 1aro). This binding sites where shown within 5.0 Angstroms radius around Mercury atom.
In total 7 binding sites of Mercury where determined in the T7 Rna Polymerase Complexed with T7 Lysozyme, PDB code: 1aro:
Jump to Mercury binding site number: 1; 2; 3; 4; 5; 6; 7;

Mercury binding site 1 out of 7 in 1aro

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Mercury binding site 1 out of 7 in the T7 Rna Polymerase Complexed with T7 Lysozyme


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 1 of T7 Rna Polymerase Complexed with T7 Lysozyme within 5.0Å range:
probe atom residue distance (Å) B Occ
L:Hg903

b:0.1
occ:1.00
O L:CYS1018 1.8 70.0 1.0
SG L:CYS1018 2.2 71.0 1.0
SG L:CYS1130 2.3 84.1 1.0
C L:CYS1018 2.8 72.1 1.0
CA L:CYS1018 3.4 69.8 1.0
CB L:CYS1018 3.4 73.5 1.0
N L:CYS1018 3.5 64.9 1.0
CB L:CYS1130 3.5 88.4 1.0
O L:SER1019 3.7 82.8 1.0
CA L:CYS1130 3.8 89.8 1.0
N L:SER1019 4.0 73.7 1.0
C L:SER1019 4.0 81.3 1.0
O L:ALA1129 4.1 97.4 1.0
N L:CYS1130 4.4 94.1 1.0
CA L:SER1019 4.4 76.7 1.0
C L:ALA1129 4.5 97.1 1.0
C L:HIS1017 4.7 62.0 1.0
N L:ALA1020 4.7 84.6 1.0
CD2 L:HIS1017 4.7 45.3 1.0
C L:PRO1131 4.9 84.5 1.0
NE2 L:HIS1017 4.9 40.0 1.0
CG L:HIS1017 4.9 53.7 1.0
CA L:PRO1131 5.0 84.2 1.0
O L:PRO1131 5.0 82.9 1.0

Mercury binding site 2 out of 7 in 1aro

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Mercury binding site 2 out of 7 in the T7 Rna Polymerase Complexed with T7 Lysozyme


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 2 of T7 Rna Polymerase Complexed with T7 Lysozyme within 5.0Å range:
probe atom residue distance (Å) B Occ
P:Hg904

b:87.6
occ:1.00
SG P:CYS125 1.5 44.8 1.0
CB P:CYS125 3.3 52.0 1.0
CB P:ALA140 3.4 60.0 1.0
CA P:CYS125 4.1 53.9 1.0
O P:ALA136 4.1 62.0 1.0
C P:ALA136 4.2 63.3 1.0
N P:VAL137 4.3 62.9 1.0
CA P:VAL137 4.4 63.0 1.0
CB P:ALA136 4.6 63.0 1.0
CA P:ALA140 4.7 61.5 1.0
CA P:ALA136 4.9 64.9 1.0
N P:CYS125 5.0 54.1 1.0

Mercury binding site 3 out of 7 in 1aro

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Mercury binding site 3 out of 7 in the T7 Rna Polymerase Complexed with T7 Lysozyme


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 3 of T7 Rna Polymerase Complexed with T7 Lysozyme within 5.0Å range:
probe atom residue distance (Å) B Occ
P:Hg905

b:83.1
occ:1.00
SG P:CYS530 1.8 27.7 1.0
CD2 P:TYR528 3.2 88.1 1.0
CB P:CYS530 3.3 52.0 1.0
OE2 P:GLU517 3.7 46.8 1.0
CE2 P:TYR528 3.7 90.3 1.0
CA P:CYS530 3.8 57.7 1.0
CD P:GLU517 3.8 52.7 1.0
O P:GLU517 4.0 64.1 1.0
CG P:GLU517 4.1 51.4 1.0
CG2 P:VAL521 4.4 74.9 1.0
OE1 P:GLU517 4.4 54.2 1.0
CG P:TYR528 4.4 85.6 1.0
N P:VAL521 4.5 72.0 1.0
C P:GLY520 4.5 71.9 1.0
CA P:GLU517 4.6 60.4 1.0
N P:CYS530 4.6 67.0 1.0
C P:GLU517 4.7 63.5 1.0
CB P:TYR528 4.7 78.7 1.0
CA P:GLY520 4.7 71.6 1.0
O P:GLY520 4.8 73.2 1.0
CA P:VAL521 4.9 74.5 1.0
CB P:GLU517 5.0 55.2 1.0

Mercury binding site 4 out of 7 in 1aro

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Mercury binding site 4 out of 7 in the T7 Rna Polymerase Complexed with T7 Lysozyme


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 4 of T7 Rna Polymerase Complexed with T7 Lysozyme within 5.0Å range:
probe atom residue distance (Å) B Occ
P:Hg906

b:0.6
occ:1.00
O P:CYS510 2.6 68.5 1.0
SG P:CYS467 2.8 72.2 1.0
CB P:CYS467 3.3 63.4 1.0
CB P:CYS510 3.3 68.5 1.0
C P:CYS510 3.5 68.4 1.0
CA P:CYS467 3.8 59.0 1.0
SG P:CYS510 3.8 57.4 1.0
CA P:CYS510 3.9 67.0 1.0
NE2 P:HIS463 4.0 54.5 1.0
OG P:SER507 4.3 84.7 1.0
CE1 P:HIS463 4.3 55.3 1.0
N P:PHE511 4.5 65.1 1.0
N P:CYS467 4.7 55.6 1.0
CD2 P:HIS463 4.9 53.9 1.0
C P:CYS467 4.9 59.1 1.0
O P:CYS467 4.9 59.3 1.0
CA P:PHE511 5.0 64.3 1.0

Mercury binding site 5 out of 7 in 1aro

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Mercury binding site 5 out of 7 in the T7 Rna Polymerase Complexed with T7 Lysozyme


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 5 of T7 Rna Polymerase Complexed with T7 Lysozyme within 5.0Å range:
probe atom residue distance (Å) B Occ
P:Hg907

b:0.4
occ:1.00
CE P:MET313 1.4 75.4 1.0
CG P:MET313 2.2 67.9 1.0
SD P:MET313 2.3 86.8 1.0
CB P:MET313 3.8 59.0 1.0
CE1 P:TYR317 3.9 40.8 1.0
CB P:VAL316 4.2 42.9 1.0
CG2 P:VAL316 4.2 33.6 1.0
CG1 P:VAL311 4.3 46.1 1.0
O P:MET313 4.3 54.0 1.0
CG1 P:VAL316 4.3 40.5 1.0
CD1 P:PHE733 4.5 43.2 1.0
CZ P:TYR317 4.5 41.8 1.0
OH P:TYR317 4.6 38.2 1.0
CD1 P:TYR317 4.6 38.8 1.0
CA P:MET313 4.7 54.1 1.0
CD2 P:TYR308 4.7 53.0 1.0
N P:MET313 4.7 52.4 1.0
CD P:PRO734 4.7 34.6 1.0
CD1 P:LEU296 4.7 28.4 1.0
O P:VAL311 4.9 44.9 1.0
CB P:VAL311 4.9 48.5 1.0
C P:MET313 4.9 53.4 1.0
CA P:PHE733 5.0 37.2 1.0

Mercury binding site 6 out of 7 in 1aro

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Mercury binding site 6 out of 7 in the T7 Rna Polymerase Complexed with T7 Lysozyme


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 6 of T7 Rna Polymerase Complexed with T7 Lysozyme within 5.0Å range:
probe atom residue distance (Å) B Occ
P:Hg908

b:0.9
occ:1.00
CG P:MET401 1.3 78.6 1.0
CB P:MET401 1.5 75.3 1.0
SD P:MET401 2.4 85.4 1.0
CE P:MET401 2.4 82.0 1.0
CA P:MET401 3.0 71.1 1.0
N P:MET401 3.6 68.6 1.0
O P:SER397 3.7 60.1 1.0
OG1 P:THR440 4.1 59.7 1.0
CD1 P:PHE432 4.1 46.4 1.0
C P:MET401 4.1 70.4 1.0
CG2 P:THR440 4.3 50.3 1.0
O P:MET401 4.3 70.4 1.0
C P:PHE400 4.6 68.3 1.0
OG P:SER397 4.6 56.2 1.0
CE1 P:PHE432 4.8 47.9 1.0
C P:SER397 4.8 61.5 1.0
CA P:PHE432 4.8 47.2 1.0
CE P:MET431 4.8 44.6 1.0
CB P:THR440 4.9 56.6 1.0
O P:LEU398 4.9 66.8 1.0
CB P:PHE432 5.0 47.9 1.0

Mercury binding site 7 out of 7 in 1aro

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Mercury binding site 7 out of 7 in the T7 Rna Polymerase Complexed with T7 Lysozyme


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 7 of T7 Rna Polymerase Complexed with T7 Lysozyme within 5.0Å range:
probe atom residue distance (Å) B Occ
P:Hg909

b:0.9
occ:1.00
SG P:CYS540 2.7 91.4 1.0
CB P:CYS540 3.3 90.5 1.0
CA P:CYS540 3.4 90.8 1.0
O P:SER539 3.7 95.8 1.0
CB P:MET635 3.8 75.5 1.0
OH P:TYR571 4.0 94.8 1.0
O P:MET635 4.2 74.2 1.0
N P:CYS540 4.3 92.9 1.0
C P:SER539 4.4 93.0 1.0
CA P:MET635 4.4 76.0 1.0
N P:SER541 4.5 87.2 1.0
C P:CYS540 4.5 88.8 1.0
CE P:MET635 4.6 74.7 1.0
C P:MET635 4.8 74.8 1.0
CG P:MET635 5.0 74.5 1.0

Reference:

D.Jeruzalmi, T.A.Steitz. Structure of T7 Rna Polymerase Complexed to the Transcriptional Inhibitor T7 Lysozyme. Embo J. V. 17 4101 1998.
ISSN: ISSN 0261-4189
PubMed: 9670025
DOI: 10.1093/EMBOJ/17.14.4101
Page generated: Sun Dec 13 19:01:59 2020

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