Atomistry » Mercury » PDB 1of5-1rsr » 1piz
Atomistry »
  Mercury »
    PDB 1of5-1rsr »
      1piz »

Mercury in PDB 1piz: Ribonucleotide Reductase R2 D84E Mutant Soaked with Ferrous Ions at Neutral pH

Enzymatic activity of Ribonucleotide Reductase R2 D84E Mutant Soaked with Ferrous Ions at Neutral pH

All present enzymatic activity of Ribonucleotide Reductase R2 D84E Mutant Soaked with Ferrous Ions at Neutral pH:
1.17.4.1;

Protein crystallography data

The structure of Ribonucleotide Reductase R2 D84E Mutant Soaked with Ferrous Ions at Neutral pH, PDB code: 1piz was solved by W.C.Voegtli, M.Sommerhalter, L.Saleh, J.Baldwin, J.M.Bollinger Jr., A.C.Rosenzweig, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 23.85 / 1.90
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 74.000, 84.200, 114.200, 90.00, 90.00, 90.00
R / Rfree (%) 20.7 / 24.2

Other elements in 1piz:

The structure of Ribonucleotide Reductase R2 D84E Mutant Soaked with Ferrous Ions at Neutral pH also contains other interesting chemical elements:

Iron (Fe) 4 atoms

Mercury Binding Sites:

Pages:

>>> Page 1 <<< Page 2, Binding sites: 11 - 11;

Binding sites:

The binding sites of Mercury atom in the Ribonucleotide Reductase R2 D84E Mutant Soaked with Ferrous Ions at Neutral pH (pdb code 1piz). This binding sites where shown within 5.0 Angstroms radius around Mercury atom.
In total 11 binding sites of Mercury where determined in the Ribonucleotide Reductase R2 D84E Mutant Soaked with Ferrous Ions at Neutral pH, PDB code: 1piz:
Jump to Mercury binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9; 10;

Mercury binding site 1 out of 11 in 1piz

Go back to Mercury Binding Sites List in 1piz
Mercury binding site 1 out of 11 in the Ribonucleotide Reductase R2 D84E Mutant Soaked with Ferrous Ions at Neutral pH


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 1 of Ribonucleotide Reductase R2 D84E Mutant Soaked with Ferrous Ions at Neutral pH within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Hg378

b:20.5
occ:1.00
 SG A:CYS272 2.4 14.4 1.0
OH A:TYR194 2.8 15.3 1.0
CB A:CYS272 3.2 14.2 1.0
O A:ALA265 3.5 22.2 1.0
CZ A:TYR194 3.8 15.5 1.0
CA A:LYS269 3.8 21.6 1.0
CE2 A:TYR194 3.8 15.4 1.0
CE A:MET198 4.0 14.2 1.0
CD2 A:LEU321 4.1 12.7 1.0
CB A:LYS269 4.1 22.8 1.0
C A:ALA265 4.4 22.0 1.0
N A:LYS269 4.4 22.5 1.0
O A:HOH410 4.5 21.0 1.0
O A:CYS268 4.7 22.9 1.0
O A:LYS269 4.7 18.6 1.0
CA A:ALA265 4.7 20.8 1.0
CA A:CYS272 4.7 14.0 1.0
C A:LYS269 4.7 20.1 1.0
C A:CYS268 4.8 24.1 1.0
CB A:ALA265 4.8 20.4 1.0
CG A:LEU321 4.9 12.9 1.0
CG A:MET198 5.0 14.1 1.0

Mercury binding site 2 out of 11 in 1piz

Go back to Mercury Binding Sites List in 1piz
Mercury binding site 2 out of 11 in the Ribonucleotide Reductase R2 D84E Mutant Soaked with Ferrous Ions at Neutral pH


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 2 of Ribonucleotide Reductase R2 D84E Mutant Soaked with Ferrous Ions at Neutral pH within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Hg379

b:19.0
occ:1.00
 SG A:CYS196 2.3 15.7 1.0
O A:CYS196 3.1 12.6 1.0
CB A:CYS196 3.3 13.6 1.0
CA A:CYS196 3.4 12.2 1.0
CD1 A:TYR157 3.5 12.1 1.0
CE1 A:TYR157 3.5 12.9 1.0
C A:CYS196 3.6 12.2 1.0
CG A:TYR157 3.9 13.0 1.0
CZ A:TYR157 3.9 12.9 1.0
CB A:TYR156 4.1 23.6 1.0
CD2 A:TYR157 4.2 12.6 1.0
CE2 A:TYR157 4.2 12.5 1.0
CB A:SER199 4.2 11.2 1.0
N A:TYR157 4.2 17.1 1.0
CD1 A:TYR156 4.2 28.8 1.0
CG A:TYR156 4.4 27.2 1.0
CA A:TYR157 4.5 15.2 1.0
C A:TYR156 4.6 18.8 1.0
OH A:TYR157 4.6 13.4 1.0
N A:VAL200 4.7 9.7 1.0
O A:ILE153 4.7 15.7 1.0
CB A:TYR157 4.7 14.5 1.0
N A:LEU197 4.8 11.7 1.0
N A:CYS196 4.8 11.3 1.0
OG A:SER199 4.8 12.2 1.0
CB A:VAL200 5.0 11.2 1.0

Mercury binding site 3 out of 11 in 1piz

Go back to Mercury Binding Sites List in 1piz
Mercury binding site 3 out of 11 in the Ribonucleotide Reductase R2 D84E Mutant Soaked with Ferrous Ions at Neutral pH


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 3 of Ribonucleotide Reductase R2 D84E Mutant Soaked with Ferrous Ions at Neutral pH within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Hg380

b:28.9
occ:1.00
 SG A:CYS214 2.3 19.4 1.0
O A:VAL210 2.8 14.1 1.0
N A:CYS214 3.3 15.2 1.0
CB A:CYS214 3.5 18.0 1.0
CA A:CYS214 3.6 16.3 1.0
CB A:ALA213 3.8 14.3 1.0
CD1 A:LEU299 3.9 19.3 1.0
C A:VAL210 4.0 14.7 1.0
C A:ALA213 4.0 14.6 1.0
CG1 A:VAL210 4.0 15.8 1.0
CD1 A:LEU304 4.2 18.5 1.0
CA A:ALA213 4.4 14.6 1.0
CG A:LEU304 4.5 17.8 1.0
O A:HOH459 4.5 36.0 1.0
CA A:VAL210 4.8 14.9 1.0
O A:ALA213 4.8 14.3 1.0
CB A:LEU304 4.9 17.6 1.0
N A:ALA213 4.9 14.8 1.0
N A:SER211 5.0 14.1 1.0

Mercury binding site 4 out of 11 in 1piz

Go back to Mercury Binding Sites List in 1piz
Mercury binding site 4 out of 11 in the Ribonucleotide Reductase R2 D84E Mutant Soaked with Ferrous Ions at Neutral pH


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 4 of Ribonucleotide Reductase R2 D84E Mutant Soaked with Ferrous Ions at Neutral pH within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Hg381

b:29.3
occ:0.50
 SG A:CYS305 2.3 25.6 1.0
CB A:CYS305 3.4 19.6 1.0
CD A:GLU309 3.4 15.7 1.0
OE2 A:GLU309 3.5 16.7 1.0
C A:CYS305 3.6 17.6 1.0
O A:CYS305 3.7 17.0 1.0
OE1 A:GLU309 3.7 16.2 1.0
N A:GLN306 3.7 16.8 1.0
CG A:GLU309 3.9 15.0 1.0
CA A:GLN306 4.1 16.4 1.0
CA A:CYS305 4.1 18.4 1.0
NZ A:LYS284 4.4 17.9 1.0
CG A:GLN306 4.4 18.6 1.0
CE A:LYS284 4.4 16.5 1.0
O A:HOH493 4.5 21.0 1.0
NH2 A:ARG328 4.7 35.4 1.0
CB A:GLN306 4.8 17.4 1.0
O A:ASP302 4.9 20.1 1.0

Mercury binding site 5 out of 11 in 1piz

Go back to Mercury Binding Sites List in 1piz
Mercury binding site 5 out of 11 in the Ribonucleotide Reductase R2 D84E Mutant Soaked with Ferrous Ions at Neutral pH


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 5 of Ribonucleotide Reductase R2 D84E Mutant Soaked with Ferrous Ions at Neutral pH within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Hg382

b:91.4
occ:0.50
 SG A:CYS268 2.5 34.2 1.0
CB A:CYS268 2.7 27.8 1.0
O A:ILE264 3.3 16.5 1.0
CG2 A:ILE264 3.4 15.9 1.0
N A:CYS268 3.4 26.1 1.0
CA A:CYS268 3.6 26.2 1.0
CD A:LYS191 3.7 17.7 1.0
CG A:LYS191 3.7 16.3 1.0
CE A:LYS191 4.1 19.5 1.0
C A:ILE264 4.1 17.6 1.0
CB A:GLU267 4.2 29.0 1.0
C A:GLU267 4.2 26.8 1.0
CA A:ILE264 4.2 16.9 1.0
CB A:LYS191 4.2 14.1 1.0
CB A:ILE264 4.3 16.2 1.0
NZ A:LYS191 4.7 20.7 1.0
OE1 A:GLU267 4.7 34.6 1.0
CA A:GLU267 4.8 27.1 1.0
CG1 A:ILE264 4.8 15.8 1.0
O A:GLU267 4.9 26.7 1.0
C A:CYS268 4.9 24.1 1.0
CA A:LYS191 4.9 12.2 1.0

Mercury binding site 6 out of 11 in 1piz

Go back to Mercury Binding Sites List in 1piz
Mercury binding site 6 out of 11 in the Ribonucleotide Reductase R2 D84E Mutant Soaked with Ferrous Ions at Neutral pH


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 6 of Ribonucleotide Reductase R2 D84E Mutant Soaked with Ferrous Ions at Neutral pH within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Hg378

b:32.1
occ:1.00
 CB B:CYS196 2.7 8.0 1.0
CA B:CYS196 2.8 11.3 1.0
SG B:CYS196 3.0 2.0 1.0
O B:CYS196 3.2 11.3 1.0
C B:CYS196 3.4 11.3 1.0
CG2 B:VAL200 3.4 10.6 1.0
CB B:TYR156 3.5 17.2 1.0
CD1 B:TYR157 3.5 13.5 1.0
CG B:TYR157 3.7 12.9 1.0
CE1 B:TYR157 3.7 12.6 1.0
CD2 B:TYR157 3.8 12.5 1.0
N B:TYR157 3.8 13.8 1.0
CZ B:TYR157 3.9 12.7 1.0
CE2 B:TYR157 4.0 12.2 1.0
C B:TYR156 4.1 14.5 1.0
CA B:TYR157 4.2 13.0 1.0
N B:CYS196 4.2 13.2 1.0
CB B:SER199 4.3 12.6 1.0
CA B:TYR156 4.5 15.3 1.0
CB B:TYR157 4.5 12.7 1.0
O B:ILE153 4.5 16.9 1.0
OG B:SER199 4.5 14.9 1.0
N B:VAL200 4.6 9.6 1.0
N B:LEU197 4.6 12.2 1.0
O B:TYR156 4.6 13.5 1.0
CG B:TYR156 4.6 18.9 1.0
OH B:TYR157 4.7 11.1 1.0
CB B:VAL200 4.7 10.7 1.0
O B:LEU195 4.9 15.5 1.0

Mercury binding site 7 out of 11 in 1piz

Go back to Mercury Binding Sites List in 1piz
Mercury binding site 7 out of 11 in the Ribonucleotide Reductase R2 D84E Mutant Soaked with Ferrous Ions at Neutral pH


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 7 of Ribonucleotide Reductase R2 D84E Mutant Soaked with Ferrous Ions at Neutral pH within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Hg379

b:15.4
occ:0.50
 SG B:CYS272 2.4 24.3 1.0
SG B:CYS268 2.4 28.0 1.0
O B:CYS268 2.7 31.5 1.0
CB B:CYS268 3.2 30.4 1.0
C B:CYS268 3.3 31.1 1.0
CB B:CYS272 3.4 21.2 1.0
CD2 B:TYR194 3.6 13.7 1.0
CA B:CYS268 3.9 30.9 1.0
N B:CYS272 3.9 21.4 1.0
CD2 B:LEU195 3.9 16.4 1.0
CE2 B:TYR194 4.0 14.0 1.0
CA B:CYS272 4.1 20.7 1.0
N B:LYS269 4.2 31.2 1.0
O B:ALA265 4.5 25.2 1.0
CA B:LYS269 4.5 31.2 1.0
N B:CYS268 4.7 30.8 1.0
CB B:GLU271 4.7 23.0 1.0
CG B:TYR194 4.8 13.5 1.0
C B:GLU271 4.9 22.2 1.0
CA B:ALA265 4.9 24.8 1.0

Mercury binding site 8 out of 11 in 1piz

Go back to Mercury Binding Sites List in 1piz
Mercury binding site 8 out of 11 in the Ribonucleotide Reductase R2 D84E Mutant Soaked with Ferrous Ions at Neutral pH


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 8 of Ribonucleotide Reductase R2 D84E Mutant Soaked with Ferrous Ions at Neutral pH within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Hg380

b:33.5
occ:0.50
 SG B:CYS214 2.6 8.6 1.0
O B:VAL210 2.8 16.4 1.0
CD1 B:LEU304 2.9 28.9 1.0
N B:CYS214 3.2 17.4 1.0
CG B:LEU304 3.4 28.2 1.0
CB B:CYS214 3.5 15.4 1.0
CA B:CYS214 3.6 16.5 1.0
CB B:ALA213 3.7 17.4 1.0
C B:VAL210 3.9 16.8 1.0
CG1 B:VAL210 3.9 19.1 1.0
C B:ALA213 4.0 17.1 1.0
CB B:LEU304 4.1 26.7 1.0
CD1 B:LEU299 4.2 39.7 1.0
CA B:ALA213 4.4 16.9 1.0
CD2 B:LEU299 4.4 39.7 1.0
CA B:VAL210 4.6 17.0 1.0
CD2 B:LEU304 4.6 28.8 1.0
N B:ALA213 4.7 16.6 1.0
O B:ALA213 4.8 17.1 1.0
CG B:LEU299 4.8 39.1 1.0
CA B:LEU304 4.9 25.9 1.0
N B:SER211 4.9 16.3 1.0
CB B:VAL210 4.9 17.7 1.0

Mercury binding site 9 out of 11 in 1piz

Go back to Mercury Binding Sites List in 1piz
Mercury binding site 9 out of 11 in the Ribonucleotide Reductase R2 D84E Mutant Soaked with Ferrous Ions at Neutral pH


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 9 of Ribonucleotide Reductase R2 D84E Mutant Soaked with Ferrous Ions at Neutral pH within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Hg381

b:67.9
occ:0.50
 O B:CYS272 2.6 18.6 1.0
CA B:CYS272 2.9 20.7 1.0
CB B:CYS272 2.9 21.2 1.0
C B:CYS272 3.1 19.4 1.0
CG B:MET198 3.2 18.0 1.0
CB B:MET198 3.2 15.0 1.0
SG B:CYS272 3.3 24.3 1.0
SD B:MET198 3.5 19.9 1.0
CB B:LEU275 3.5 21.5 1.0
CD1 B:LEU275 3.6 23.6 1.0
CD2 B:LEU275 3.6 23.6 1.0
CG B:LEU275 3.8 22.9 1.0
CD2 B:PHE276 4.0 20.5 1.0
N B:PHE276 4.2 19.9 1.0
N B:CYS272 4.3 21.4 1.0
N B:TYR273 4.3 18.6 1.0
CA B:MET198 4.5 12.7 1.0
CA B:LEU275 4.6 20.1 1.0
CD1 B:LEU321 4.6 21.6 1.0
C B:MET198 4.6 11.6 1.0
C B:LEU275 4.7 20.1 1.0
CE2 B:PHE276 4.7 19.9 1.0
CB B:PHE276 4.7 19.9 1.0
CG B:PHE276 4.8 19.5 1.0
O B:GLU271 4.8 21.8 1.0
O B:MET198 4.8 11.1 1.0
N B:LEU275 4.9 19.7 1.0
CE B:MET198 4.9 20.3 1.0
CA B:PHE276 4.9 19.1 1.0
C B:GLU271 5.0 22.2 1.0

Mercury binding site 10 out of 11 in 1piz

Go back to Mercury Binding Sites List in 1piz
Mercury binding site 10 out of 11 in the Ribonucleotide Reductase R2 D84E Mutant Soaked with Ferrous Ions at Neutral pH


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 10 of Ribonucleotide Reductase R2 D84E Mutant Soaked with Ferrous Ions at Neutral pH within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Hg382

b:44.4
occ:0.50
 CE B:LYS284 2.4 27.3 1.0
SG B:CYS305 2.5 28.5 1.0
NZ B:LYS284 2.6 28.4 1.0
CD B:LYS284 2.7 27.4 1.0
CG B:LYS284 2.9 26.9 1.0
O B:CYS305 3.0 22.1 1.0
HG B:HG383 3.5 56.8 0.5
CG B:GLU309 3.5 19.8 1.0
C B:CYS305 3.6 22.9 1.0
CA B:CYS305 3.6 23.8 1.0
CB B:CYS305 3.6 24.7 1.0
OE2 B:GLU309 3.9 22.1 1.0
CB B:LYS284 4.0 26.1 1.0
CD B:GLU309 4.0 21.7 1.0
CB B:VAL308 4.4 15.3 1.0
CG1 B:VAL308 4.6 15.1 1.0
N B:GLU309 4.7 16.4 1.0
N B:GLN306 4.7 21.2 1.0
CB B:GLU309 4.8 18.1 1.0
N B:CYS305 5.0 24.6 1.0

Reference:

W.C.Voegtli, M.Sommerhalter, L.Saleh, J.Baldwin, J.M.Bollinger Jr., A.C.Rosenzweig. Variable Coordination Geometries at the Diiron(II) Active Site of Ribonucleotide Reductase R2. J.Am.Chem.Soc. V. 125 15822 2003.
ISSN: ISSN 0002-7863
PubMed: 14677973
DOI: 10.1021/JA0370387
Page generated: Sun Aug 11 01:02:47 2024

Last articles

Zn in 9JYW
Zn in 9IR4
Zn in 9IR3
Zn in 9GMX
Zn in 9GMW
Zn in 9JEJ
Zn in 9ERF
Zn in 9ERE
Zn in 9EGV
Zn in 9EGW
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy