Atomistry » Mercury » PDB 1rsv-1yp2 » 1wt2
Atomistry »
  Mercury »
    PDB 1rsv-1yp2 »
      1wt2 »

Mercury in PDB 1wt2: Mutant Human Abo(H) Blood Group Glycosyltransferase A with Bound Udp and Inhibitor

Enzymatic activity of Mutant Human Abo(H) Blood Group Glycosyltransferase A with Bound Udp and Inhibitor

All present enzymatic activity of Mutant Human Abo(H) Blood Group Glycosyltransferase A with Bound Udp and Inhibitor:
2.4.1.40;

Protein crystallography data

The structure of Mutant Human Abo(H) Blood Group Glycosyltransferase A with Bound Udp and Inhibitor, PDB code: 1wt2 was solved by H.J.Lee, C.H.Barry, S.N.Borisova, N.O.L.Seto, R.B.Zheng, A.Blancher, S.V.Evans, M.M.Palcic, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 20.00 / 1.90
Space group C 2 2 21
Cell size a, b, c (Å), α, β, γ (°) 52.800, 150.100, 79.800, 90.00, 90.00, 90.00
R / Rfree (%) 21 / 25.3

Mercury Binding Sites:

The binding sites of Mercury atom in the Mutant Human Abo(H) Blood Group Glycosyltransferase A with Bound Udp and Inhibitor (pdb code 1wt2). This binding sites where shown within 5.0 Angstroms radius around Mercury atom.
In total 4 binding sites of Mercury where determined in the Mutant Human Abo(H) Blood Group Glycosyltransferase A with Bound Udp and Inhibitor, PDB code: 1wt2:
Jump to Mercury binding site number: 1; 2; 3; 4;

Mercury binding site 1 out of 4 in 1wt2

Go back to Mercury Binding Sites List in 1wt2
Mercury binding site 1 out of 4 in the Mutant Human Abo(H) Blood Group Glycosyltransferase A with Bound Udp and Inhibitor


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 1 of Mutant Human Abo(H) Blood Group Glycosyltransferase A with Bound Udp and Inhibitor within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Hg401

b:41.5
occ:0.50
SD A:MET288 2.9 38.0 1.0
OD1 A:ASP302 3.1 39.4 1.0
O A:CYS284 3.6 28.3 1.0
C A:CYS284 3.7 27.4 1.0
CB A:CYS284 3.8 26.5 1.0
N A:HIS285 3.8 28.3 1.0
CG A:MET288 3.8 32.9 1.0
CA A:HIS285 3.9 31.0 1.0
CG A:ASP302 4.3 36.8 1.0
CA A:CYS284 4.4 26.5 1.0
CE A:MET288 4.5 37.0 1.0
CB A:MET288 4.6 31.5 1.0
CB A:HIS285 4.6 33.0 1.0
O A:THR281 4.8 24.6 1.0
HG A:HG402 4.8 54.6 0.5
CB A:ASP302 4.9 33.2 1.0

Mercury binding site 2 out of 4 in 1wt2

Go back to Mercury Binding Sites List in 1wt2
Mercury binding site 2 out of 4 in the Mutant Human Abo(H) Blood Group Glycosyltransferase A with Bound Udp and Inhibitor


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 2 of Mutant Human Abo(H) Blood Group Glycosyltransferase A with Bound Udp and Inhibitor within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Hg402

b:54.6
occ:0.50
SG A:CYS284 2.6 34.7 1.0
CB A:CYS284 3.1 26.5 1.0
CD2 A:LEU280 3.4 22.6 1.0
CB A:LEU306 3.7 19.6 1.0
CA A:LEU306 3.8 19.0 1.0
O A:ASP302 3.9 24.9 1.0
N A:LEU306 3.9 17.8 1.0
CD1 A:LEU306 4.4 23.3 1.0
CG A:LEU306 4.5 21.8 1.0
CG A:LEU280 4.5 19.2 1.0
C A:HIS305 4.6 19.4 1.0
O A:LEU280 4.6 21.0 1.0
CA A:CYS284 4.7 26.5 1.0
C A:ASP302 4.7 26.1 1.0
CB A:HIS305 4.8 22.2 1.0
HG A:HG401 4.8 41.5 0.5
CB A:ASP302 5.0 33.2 1.0

Mercury binding site 3 out of 4 in 1wt2

Go back to Mercury Binding Sites List in 1wt2
Mercury binding site 3 out of 4 in the Mutant Human Abo(H) Blood Group Glycosyltransferase A with Bound Udp and Inhibitor


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 3 of Mutant Human Abo(H) Blood Group Glycosyltransferase A with Bound Udp and Inhibitor within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Hg403

b:42.0
occ:1.00
SG A:CYS209 2.5 28.6 1.0
OG1 A:THR119 2.9 20.1 1.0
CB A:CYS209 3.2 19.3 1.0
CB A:THR119 3.9 20.2 1.0
CG2 A:THR119 4.0 20.5 1.0
O A:HOH1168 4.1 28.9 1.0
CA A:CYS209 4.2 17.5 1.0
CG1 A:VAL277 4.3 19.4 1.0
N A:CYS209 4.7 15.2 1.0
CD1 A:LEU207 4.8 23.0 1.0
CZ A:PHE270 4.8 21.0 1.0
CE1 A:PHE270 5.0 20.4 1.0

Mercury binding site 4 out of 4 in 1wt2

Go back to Mercury Binding Sites List in 1wt2
Mercury binding site 4 out of 4 in the Mutant Human Abo(H) Blood Group Glycosyltransferase A with Bound Udp and Inhibitor


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 4 of Mutant Human Abo(H) Blood Group Glycosyltransferase A with Bound Udp and Inhibitor within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Hg404

b:36.3
occ:0.50
SG A:CYS284 2.6 34.7 1.0
O A:HIS305 3.1 18.8 1.0
O A:HOH1286 3.3 31.9 1.0
CA A:CYS284 3.3 26.5 1.0
CB A:CYS284 3.5 26.5 1.0
N A:CYS284 3.5 24.1 1.0
CB A:TYR309 3.6 16.8 1.0
C A:HIS305 3.7 19.4 1.0
CB A:HIS305 3.8 22.2 1.0
C A:ALA283 3.9 23.8 1.0
CD1 A:TYR309 4.1 18.1 1.0
O A:ALA283 4.1 24.6 1.0
CA A:HIS305 4.2 19.9 1.0
CG A:TYR309 4.2 17.1 1.0
N A:TYR309 4.3 16.6 1.0
CA A:TYR309 4.3 18.1 1.0
CB A:ALA283 4.4 23.4 1.0
O A:LEU280 4.6 21.0 1.0
N A:LEU306 4.6 17.8 1.0
O A:HOH1238 4.6 37.8 1.0
CB A:ALA287 4.6 29.1 1.0
C A:CYS284 4.7 27.4 1.0
CG A:HIS305 4.7 24.1 1.0
CA A:ALA283 4.8 23.9 1.0
CD2 A:HIS305 4.9 23.5 1.0
CA A:LEU306 4.9 19.0 1.0

Reference:

H.J.Lee, C.H.Barry, S.N.Borisova, N.O.L.Seto, R.B.Zheng, A.Blancher, S.V.Evans, M.M.Palcic. Structural Basis For the Inactivity of Human Blood Group O2 Glycosyltransferase J.Biol.Chem. V. 280 525 2005.
ISSN: ISSN 0021-9258
PubMed: 15475562
DOI: 10.1074/JBC.M410245200
Page generated: Wed Oct 28 18:40:51 2020

Last articles

Xe in 6AYK
Xe in 6QII
Xe in 6ASM
Xe in 5NSW
Xe in 6FY9
Xe in 5O1K
Xe in 5O27
Xe in 5M69
Xe in 5KPU
Xe in 5I63
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy