Atomistry » Mercury » PDB 2esw-2o1f » 2j4f
Atomistry »
  Mercury »
    PDB 2esw-2o1f »
      2j4f »

Mercury in PDB 2j4f: Torpedo Acetylcholinesterase - Hg Heavy-Atom Derivative

Enzymatic activity of Torpedo Acetylcholinesterase - Hg Heavy-Atom Derivative

All present enzymatic activity of Torpedo Acetylcholinesterase - Hg Heavy-Atom Derivative:
3.1.1.7;

Protein crystallography data

The structure of Torpedo Acetylcholinesterase - Hg Heavy-Atom Derivative, PDB code: 2j4f was solved by D.I.Kreimer, E.A.Dolginova, M.Raves, J.L.Sussman, I.Silman, L.Weiner, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 8.0 / 2.8
Space group P 31 2 1
Cell size a, b, c (Å), α, β, γ (°) 110.700, 110.700, 135.000, 90.00, 90.00, 120.00
R / Rfree (%) 20.4 / 26.2

Mercury Binding Sites:

The binding sites of Mercury atom in the Torpedo Acetylcholinesterase - Hg Heavy-Atom Derivative (pdb code 2j4f). This binding sites where shown within 5.0 Angstroms radius around Mercury atom.
In total 2 binding sites of Mercury where determined in the Torpedo Acetylcholinesterase - Hg Heavy-Atom Derivative, PDB code: 2j4f:
Jump to Mercury binding site number: 1; 2;

Mercury binding site 1 out of 2 in 2j4f

Go back to Mercury Binding Sites List in 2j4f
Mercury binding site 1 out of 2 in the Torpedo Acetylcholinesterase - Hg Heavy-Atom Derivative


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 1 of Torpedo Acetylcholinesterase - Hg Heavy-Atom Derivative within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Hg1536

b:44.0
occ:0.45
ND1 A:HIS471 1.7 30.9 1.0
CE1 A:HIS471 2.4 33.4 1.0
OE2 A:GLU139 2.4 29.2 1.0
OE1 A:GLU139 2.7 25.7 1.0
CG A:HIS471 2.9 31.8 1.0
CD A:GLU139 2.9 25.9 1.0
O A:HOH2088 3.3 37.1 1.0
NE2 A:HIS471 3.5 35.2 1.0
CB A:HIS471 3.6 30.4 1.0
CD2 A:HIS471 3.8 32.9 1.0
CA A:HIS471 4.2 28.0 1.0
CG A:GLU139 4.4 23.1 1.0
O A:THR138 4.7 23.4 1.0
O A:HOH2089 4.9 18.2 1.0

Mercury binding site 2 out of 2 in 2j4f

Go back to Mercury Binding Sites List in 2j4f
Mercury binding site 2 out of 2 in the Torpedo Acetylcholinesterase - Hg Heavy-Atom Derivative


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 2 of Torpedo Acetylcholinesterase - Hg Heavy-Atom Derivative within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Hg1537

b:30.4
occ:0.65
N A:SER228 2.3 12.3 1.0
SG A:CYS231 2.3 24.5 1.0
O A:SER228 2.3 19.4 1.0
OG A:SER228 2.4 15.3 1.0
C A:GLY227 3.0 12.4 1.0
CA A:SER228 3.0 14.0 1.0
C A:SER228 3.0 15.1 1.0
CB A:SER228 3.2 11.2 1.0
CB A:CYS231 3.2 18.2 1.0
CA A:GLY227 3.3 11.5 1.0
O A:GLY227 3.9 13.7 1.0
CZ2 A:TRP233 4.1 13.6 1.0
CA A:CYS231 4.2 17.2 1.0
N A:CYS231 4.2 17.5 1.0
N A:PRO229 4.3 14.2 1.0
N A:GLY227 4.4 10.5 1.0
CB A:ALA204 4.6 12.8 1.0
CB A:PRO403 4.7 10.1 1.0
O A:ASN399 4.7 16.6 1.0
OD1 A:ASN230 4.7 5.2 1.0
CE2 A:TRP233 4.7 13.0 1.0
CH2 A:TRP233 4.8 12.5 1.0
O A:VAL400 4.9 16.6 1.0
O A:SER226 4.9 13.6 1.0
NE1 A:TRP233 5.0 14.4 1.0

Reference:

D.I.Kreimer, E.A.Dolginova, M.Raves, J.L.Sussman, I.Silman, L.Weiner. A Metastable State of Torpedo Californica Acetylcholinesterase Generated By Modification with Organomercurials Biochemistry V. 33 14407 1994.
ISSN: ISSN 0006-2960
PubMed: 7981200
DOI: 10.1021/BI00252A006
Page generated: Wed Oct 28 18:41:41 2020

Last articles

Xe in 6AYK
Xe in 6QII
Xe in 6ASM
Xe in 5NSW
Xe in 6FY9
Xe in 5O1K
Xe in 5O27
Xe in 5M69
Xe in 5KPU
Xe in 5I63
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy