Mercury in PDB 2o1h: Naturally Occurring Mutation of Humna Abo(H) Galactosyltransferase in Complex with Udp: Gtb/M214T_UDP

The structure of Naturally Occurring Mutation of Humna Abo(H) Galactosyltransferase in Complex with Udp: Gtb/M214T_UDP, PDB code: 2o1h was solved by J.A.Letts, S.N.Borisova, S.V.Evans, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 1.67
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	52.600, 149.490, 79.240, 90.00, 90.00, 90.00
R / Rfree (%)	17.6 / 22.2

The structure of Naturally Occurring Mutation of Humna Abo(H) Galactosyltransferase in Complex with Udp: Gtb/M214T_UDP also contains other interesting chemical elements:

Manganese

(Mn)

1 atom

The binding sites of Mercury atom in the Naturally Occurring Mutation of Humna Abo(H) Galactosyltransferase in Complex with Udp: Gtb/M214T_UDP (pdb code 2o1h). This binding sites where shown within 5.0 Angstroms radius around Mercury atom.
In total 5 binding sites of Mercury where determined in the Naturally Occurring Mutation of Humna Abo(H) Galactosyltransferase in Complex with Udp: Gtb/M214T_UDP, PDB code: 2o1h:
Jump to Mercury binding site number: 1; 2; 3; 4; 5;

Mercury binding site 1 out of 5 in the Naturally Occurring Mutation of Humna Abo(H) Galactosyltransferase in Complex with Udp: Gtb/M214T_UDP


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 1 of Naturally Occurring Mutation of Humna Abo(H) Galactosyltransferase in Complex with Udp: Gtb/M214T_UDP within 5.0Å range: probe atom residue distance (Å) B Occ A:Hg401 b:24.3 occ:0.50 SG A:CYS284 2.4 14.6 0.5 O A:HOH572 2.4 26.4 1.0 O A:HIS305 3.2 16.1 1.0 CA A:CYS284 3.2 19.9 1.0 N A:CYS284 3.4 19.6 1.0 O A:HOH570 3.4 33.3 1.0 CB A:CYS284 3.4 19.9 0.5 CB A:CYS284 3.4 21.1 0.5 O A:HOH718 3.6 43.5 1.0 CB A:TYR309 3.6 15.5 1.0 C A:HIS305 3.8 17.4 1.0 C A:ALA283 3.9 20.3 1.0 CB A:HIS305 4.0 19.1 1.0 CD1 A:TYR309 4.0 14.8 1.0 O A:ALA283 4.1 21.4 1.0 CG A:TYR309 4.2 13.3 1.0 N A:TYR309 4.3 16.7 1.0 CA A:HIS305 4.3 18.0 1.0 CA A:TYR309 4.3 15.3 1.0 CB A:ALA283 4.3 17.6 1.0 O A:LEU280 4.5 18.0 1.0 C A:CYS284 4.6 21.1 1.0 N A:LEU306 4.7 15.8 1.0 SG A:CYS284 4.7 26.6 0.5 HG A:HG403 4.7 33.5 0.5 CA A:ALA283 4.8 19.4 1.0 CG A:HIS305 4.8 22.6 1.0 CD2 A:HIS305 4.8 22.9 1.0 CB A:ALA287 4.8 25.1 1.0

Mercury binding site 2 out of 5 in the Naturally Occurring Mutation of Humna Abo(H) Galactosyltransferase in Complex with Udp: Gtb/M214T_UDP


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 2 of Naturally Occurring Mutation of Humna Abo(H) Galactosyltransferase in Complex with Udp: Gtb/M214T_UDP within 5.0Å range: probe atom residue distance (Å) B Occ A:Hg402 b:42.8 occ:0.20 SD A:MET288 2.2 36.6 1.0 SG A:CYS284 2.6 26.6 0.5 CE A:MET288 3.4 35.2 1.0 C A:CYS284 3.8 21.1 1.0 CG A:MET288 3.8 28.1 1.0 N A:HIS285 3.8 20.8 1.0 CB A:CYS284 3.8 19.9 0.5 CB A:CYS284 3.8 21.1 0.5 O A:CYS284 3.9 23.1 1.0 CA A:HIS285 4.0 24.1 1.0 OD2 A:ASP302 4.3 29.5 0.5 CA A:CYS284 4.4 19.9 1.0 CG A:ASP302 4.5 26.9 0.5 CB A:MET288 4.5 26.8 1.0 CB A:HIS285 4.6 25.1 1.0 OD1 A:ASP302 4.7 25.7 0.5 O A:THR281 4.9 20.7 1.0 CB A:ASP302 4.9 26.2 0.5 HG A:HG403 4.9 33.5 0.5

Mercury binding site 3 out of 5 in the Naturally Occurring Mutation of Humna Abo(H) Galactosyltransferase in Complex with Udp: Gtb/M214T_UDP


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 3 of Naturally Occurring Mutation of Humna Abo(H) Galactosyltransferase in Complex with Udp: Gtb/M214T_UDP within 5.0Å range: probe atom residue distance (Å) B Occ A:Hg403 b:33.5 occ:0.50 SG A:CYS284 2.4 14.6 0.5 SG A:CYS284 2.9 26.6 0.5 CB A:CYS284 2.9 21.1 0.5 CB A:CYS284 3.0 19.9 0.5 CD2 A:LEU280 3.4 19.9 1.0 N A:LEU306 3.7 15.8 1.0 CA A:LEU306 3.8 15.4 1.0 O A:ASP302 3.9 22.2 1.0 CB A:LEU306 4.0 15.1 1.0 C A:HIS305 4.3 17.4 1.0 CD1 A:LEU306 4.4 18.3 1.0 CA A:CYS284 4.5 19.9 1.0 CB A:HIS305 4.5 19.1 1.0 O A:HOH709 4.5 46.8 1.0 O A:LEU280 4.5 18.0 1.0 CG A:LEU280 4.6 17.6 1.0 HG A:HG401 4.7 24.3 0.5 O A:HIS305 4.8 16.1 1.0 C A:ASP302 4.9 22.8 1.0 CG A:LEU306 4.9 14.4 1.0 HG A:HG402 4.9 42.8 0.2 CB A:ASP302 5.0 26.2 0.5

Mercury binding site 4 out of 5 in the Naturally Occurring Mutation of Humna Abo(H) Galactosyltransferase in Complex with Udp: Gtb/M214T_UDP


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 4 of Naturally Occurring Mutation of Humna Abo(H) Galactosyltransferase in Complex with Udp: Gtb/M214T_UDP within 5.0Å range: probe atom residue distance (Å) B Occ A:Hg404 b:29.3 occ:0.80 O A:HOH611 1.6 22.3 1.0 SG A:CYS209 2.3 24.4 1.0 O A:HOH640 2.4 23.6 1.0 OG1 A:THR119 2.8 22.3 1.0 O A:HOH502 3.2 14.4 1.0 CB A:CYS209 3.3 19.0 1.0 CB A:THR119 3.8 18.8 1.0 CG2 A:THR119 4.0 20.3 1.0 CA A:CYS209 4.1 16.6 1.0 O A:HOH727 4.3 26.3 1.0 CG1 A:VAL277 4.3 13.4 1.0 CD1 A:LEU207 4.6 24.0 1.0 N A:CYS209 4.7 15.4 1.0 CZ A:PHE270 5.0 18.8 1.0

Mercury binding site 5 out of 5 in the Naturally Occurring Mutation of Humna Abo(H) Galactosyltransferase in Complex with Udp: Gtb/M214T_UDP


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 5 of Naturally Occurring Mutation of Humna Abo(H) Galactosyltransferase in Complex with Udp: Gtb/M214T_UDP within 5.0Å range: probe atom residue distance (Å) B Occ A:Hg405 b:29.6 occ:0.80 SG A:CYS80 2.3 34.2 1.0 O A:HOH660 2.3 30.9 1.0 O A:GLY98 2.7 22.9 1.0 O A:HOH725 2.8 39.4 1.0 CB A:CYS80 3.0 25.4 1.0 O A:HOH604 3.3 29.9 1.0 CA A:CYS80 3.3 26.8 1.0 O A:HOH720 3.4 41.9 1.0 O A:HOH585 3.5 27.0 1.0 O A:HOH560 3.8 28.4 1.0 C A:GLY98 3.9 20.8 1.0 O A:HOH672 3.9 40.2 1.0 O A:HOH555 4.1 25.2 1.0 N A:CYS80 4.3 26.7 1.0 CA A:THR99 4.3 15.8 1.0 C A:CYS80 4.5 24.5 1.0 N A:THR99 4.6 16.4 1.0 O A:THR99 4.6 19.8 1.0 C A:THR99 4.6 16.0 1.0 O A:CYS80 4.7 27.6 1.0

M.Persson, J.A.Letts, B.Hosseini-Maaf, S.N.Borisova, M.M.Palcic, S.V.Evans, M.L.Olsson. Structural Effects of Naturally Occurring Human Blood Group B Galactosyltransferase Mutations Adjacent to the Dxd Motif. J.Biol.Chem. V. 282 9564 2007.
ISSN: ISSN 0021-9258
PubMed: 17259183
DOI: 10.1074/JBC.M610998200