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Mercury in PDB 3f0p: Crystal Structure of the Mercury-Bound Form of Merb, the Organomercurial Lyase Involved in A Bacterial Mercury Resistance System

Enzymatic activity of Crystal Structure of the Mercury-Bound Form of Merb, the Organomercurial Lyase Involved in A Bacterial Mercury Resistance System

All present enzymatic activity of Crystal Structure of the Mercury-Bound Form of Merb, the Organomercurial Lyase Involved in A Bacterial Mercury Resistance System:
4.99.1.2;

Protein crystallography data

The structure of Crystal Structure of the Mercury-Bound Form of Merb, the Organomercurial Lyase Involved in A Bacterial Mercury Resistance System, PDB code: 3f0p was solved by J.Lafrance-Vanasse, M.Lefebvre, P.Di Lello, J.Sygusch, J.G.Omichinski, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 19.47 / 1.64
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 38.372, 89.203, 51.585, 90.00, 100.48, 90.00
R / Rfree (%) 18.4 / 21.3

Other elements in 3f0p:

The structure of Crystal Structure of the Mercury-Bound Form of Merb, the Organomercurial Lyase Involved in A Bacterial Mercury Resistance System also contains other interesting chemical elements:

Bromine (Br) 1 atom

Mercury Binding Sites:

The binding sites of Mercury atom in the Crystal Structure of the Mercury-Bound Form of Merb, the Organomercurial Lyase Involved in A Bacterial Mercury Resistance System (pdb code 3f0p). This binding sites where shown within 5.0 Angstroms radius around Mercury atom.
In total 2 binding sites of Mercury where determined in the Crystal Structure of the Mercury-Bound Form of Merb, the Organomercurial Lyase Involved in A Bacterial Mercury Resistance System, PDB code: 3f0p:
Jump to Mercury binding site number: 1; 2;

Mercury binding site 1 out of 2 in 3f0p

Go back to Mercury Binding Sites List in 3f0p
Mercury binding site 1 out of 2 in the Crystal Structure of the Mercury-Bound Form of Merb, the Organomercurial Lyase Involved in A Bacterial Mercury Resistance System


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 1 of Crystal Structure of the Mercury-Bound Form of Merb, the Organomercurial Lyase Involved in A Bacterial Mercury Resistance System within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Hg213

b:16.8
occ:0.23
SG A:CYS96 2.3 13.1 1.0
SG A:CYS159 2.4 19.6 1.0
O A:HOH214 2.6 30.6 1.0
OD2 A:ASP99 2.9 22.1 1.0
CG A:ASP99 3.3 18.4 1.0
CB A:CYS96 3.3 9.2 1.0
OD1 A:ASP99 3.4 23.9 1.0
CB A:CYS159 3.4 13.6 1.0
CD2 A:PHE158 3.8 13.4 1.0
CA A:CYS159 3.8 15.5 1.0
O A:HOH358 3.9 32.0 1.0
CE2 A:PHE158 3.9 13.8 1.0
N A:CYS96 4.1 11.0 1.0
CB A:TRP95 4.3 11.6 1.0
CA A:CYS96 4.3 9.2 1.0
N A:CYS159 4.4 13.5 1.0
C A:TRP95 4.4 10.2 1.0
CB A:ASP99 4.5 12.2 1.0
O A:TRP95 4.7 11.8 1.0
N A:ASP99 4.9 10.1 1.0
CA A:TRP95 5.0 9.3 1.0

Mercury binding site 2 out of 2 in 3f0p

Go back to Mercury Binding Sites List in 3f0p
Mercury binding site 2 out of 2 in the Crystal Structure of the Mercury-Bound Form of Merb, the Organomercurial Lyase Involved in A Bacterial Mercury Resistance System


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 2 of Crystal Structure of the Mercury-Bound Form of Merb, the Organomercurial Lyase Involved in A Bacterial Mercury Resistance System within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Hg214

b:18.6
occ:0.24
SG B:CYS96 2.3 15.6 1.0
SG B:CYS159 2.4 20.4 1.0
O B:HOH219 2.6 29.4 1.0
OD2 B:ASP99 3.2 24.9 1.0
CB B:CYS159 3.3 11.8 1.0
CB B:CYS96 3.3 14.0 1.0
O B:HOH239 3.4 31.7 1.0
OD1 B:ASP99 3.5 29.7 1.0
CG B:ASP99 3.5 22.0 1.0
O B:HOH243 3.7 27.4 1.0
CA B:CYS159 3.7 17.6 1.0
CD2 B:PHE158 3.8 15.1 1.0
CE2 B:PHE158 3.9 15.8 1.0
N B:CYS96 4.1 13.3 1.0
N B:CYS159 4.3 20.8 1.0
CA B:CYS96 4.3 12.3 1.0
CB B:TRP95 4.4 13.0 1.0
C B:TRP95 4.5 12.7 1.0
CB B:ASP99 4.7 16.3 1.0
O B:TRP95 4.8 14.1 1.0
C B:CYS159 5.0 15.1 1.0
N B:ASP99 5.0 13.4 1.0

Reference:

J.Lafrance-Vanasse, M.Lefebvre, P.Di Lello, J.Sygusch, J.G.Omichinski. Crystal Structures of the Organomercurial Lyase Merb in Its Free and Mercury-Bound Forms: Insights Into the Mechanism of Methylmercury Degradation J.Biol.Chem. V. 284 938 2009.
ISSN: ISSN 0021-9258
PubMed: 19004822
DOI: 10.1074/JBC.M807143200
Page generated: Sun Aug 11 03:40:52 2024

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