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Mercury in PDB 3f2h: Crystal Structure of the Mercury-Bound Form of Merb Mutant C160S, the Organomercurial Lyase Involved in A Bacterial Mercury Resistance System

Enzymatic activity of Crystal Structure of the Mercury-Bound Form of Merb Mutant C160S, the Organomercurial Lyase Involved in A Bacterial Mercury Resistance System

All present enzymatic activity of Crystal Structure of the Mercury-Bound Form of Merb Mutant C160S, the Organomercurial Lyase Involved in A Bacterial Mercury Resistance System:
4.99.1.2;

Protein crystallography data

The structure of Crystal Structure of the Mercury-Bound Form of Merb Mutant C160S, the Organomercurial Lyase Involved in A Bacterial Mercury Resistance System, PDB code: 3f2h was solved by J.Lafrance-Vanasse, M.Lefebvre, P.Di Lello, J.Sygusch, J.G.Omichinski, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 19.30 / 2.00
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 38.880, 89.169, 54.627, 90.00, 97.45, 90.00
R / Rfree (%) 19.1 / 24

Mercury Binding Sites:

The binding sites of Mercury atom in the Crystal Structure of the Mercury-Bound Form of Merb Mutant C160S, the Organomercurial Lyase Involved in A Bacterial Mercury Resistance System (pdb code 3f2h). This binding sites where shown within 5.0 Angstroms radius around Mercury atom.
In total 2 binding sites of Mercury where determined in the Crystal Structure of the Mercury-Bound Form of Merb Mutant C160S, the Organomercurial Lyase Involved in A Bacterial Mercury Resistance System, PDB code: 3f2h:
Jump to Mercury binding site number: 1; 2;

Mercury binding site 1 out of 2 in 3f2h

Go back to Mercury Binding Sites List in 3f2h
Mercury binding site 1 out of 2 in the Crystal Structure of the Mercury-Bound Form of Merb Mutant C160S, the Organomercurial Lyase Involved in A Bacterial Mercury Resistance System


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 1 of Crystal Structure of the Mercury-Bound Form of Merb Mutant C160S, the Organomercurial Lyase Involved in A Bacterial Mercury Resistance System within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Hg221

b:18.7
occ:0.35
SG A:CYS96 2.5 13.3 1.0
SG A:CYS159 2.5 19.8 1.0
O A:HOH252 2.6 25.2 1.0
OD2 A:ASP99 3.1 20.3 1.0
OD1 A:ASP99 3.2 21.7 1.0
CG A:ASP99 3.3 19.5 1.0
CB A:CYS159 3.4 11.4 1.0
CB A:CYS96 3.4 11.9 1.0
O A:HOH291 3.6 27.5 1.0
CD2 A:PHE158 3.7 13.9 1.0
CE2 A:PHE158 3.8 11.8 1.0
CA A:CYS159 3.9 15.8 1.0
N A:CYS96 4.3 11.5 1.0
CB A:TRP95 4.4 14.6 1.0
CA A:CYS96 4.5 11.8 1.0
N A:CYS159 4.5 11.1 1.0
CB A:ASP99 4.6 17.6 1.0
C A:TRP95 4.7 14.5 1.0
N A:ASP99 5.0 13.4 1.0
CG A:PHE158 5.0 13.1 1.0

Mercury binding site 2 out of 2 in 3f2h

Go back to Mercury Binding Sites List in 3f2h
Mercury binding site 2 out of 2 in the Crystal Structure of the Mercury-Bound Form of Merb Mutant C160S, the Organomercurial Lyase Involved in A Bacterial Mercury Resistance System


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 2 of Crystal Structure of the Mercury-Bound Form of Merb Mutant C160S, the Organomercurial Lyase Involved in A Bacterial Mercury Resistance System within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Hg221

b:19.2
occ:0.27
SG B:CYS96 2.4 11.9 1.0
SG B:CYS159 2.5 19.1 1.0
O B:HOH358 2.7 36.3 1.0
OD2 B:ASP99 3.1 16.0 1.0
OD1 B:ASP99 3.2 18.8 1.0
CG B:ASP99 3.4 14.8 1.0
CB B:CYS96 3.4 8.2 1.0
CB B:CYS159 3.5 14.5 1.0
CE2 B:PHE158 3.7 13.0 1.0
CD2 B:PHE158 3.7 19.3 1.0
CA B:CYS159 3.9 18.2 1.0
CB B:TRP95 4.2 13.3 1.0
N B:CYS96 4.2 8.2 1.0
CA B:CYS96 4.4 11.5 1.0
N B:CYS159 4.4 14.9 1.0
O B:HOH228 4.5 32.0 1.0
C B:TRP95 4.6 10.3 1.0
CB B:ASP99 4.7 9.6 1.0
O B:TRP95 5.0 9.2 1.0

Reference:

J.Lafrance-Vanasse, M.Lefebvre, P.Di Lello, J.Sygusch, J.G.Omichinski. Crystal Structures of the Organomercurial Lyase Merb in Its Free and Mercury-Bound Forms: Insights Into the Mechanism of Methylmercury Degradation J.Biol.Chem. V. 284 938 2009.
ISSN: ISSN 0021-9258
PubMed: 19004822
DOI: 10.1074/JBC.M807143200
Page generated: Wed Oct 28 18:42:31 2020

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