Mercury in PDB 4izm: Crystal Structure of Gltph L66C-S300C Mutant Crosslinked with Divalent Mercury

The structure of Crystal Structure of Gltph L66C-S300C Mutant Crosslinked with Divalent Mercury, PDB code: 4izm was solved by N.Reyes, O.Boudker, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 4.50
Space group	P 61
Cell size a, b, c (Å), α, β, γ (°)	119.708, 119.708, 333.720, 90.00, 90.00, 120.00
R / Rfree (%)	25 / 29.9

The structure of Crystal Structure of Gltph L66C-S300C Mutant Crosslinked with Divalent Mercury also contains other interesting chemical elements:

Sodium

(Na)

6 atoms

The binding sites of Mercury atom in the Crystal Structure of Gltph L66C-S300C Mutant Crosslinked with Divalent Mercury (pdb code 4izm). This binding sites where shown within 5.0 Angstroms radius around Mercury atom.
In total 3 binding sites of Mercury where determined in the Crystal Structure of Gltph L66C-S300C Mutant Crosslinked with Divalent Mercury, PDB code: 4izm:
Jump to Mercury binding site number: 1; 2; 3;

Mercury binding site 1 out of 3 in the Crystal Structure of Gltph L66C-S300C Mutant Crosslinked with Divalent Mercury


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 1 of Crystal Structure of Gltph L66C-S300C Mutant Crosslinked with Divalent Mercury within 5.0Å range: probe atom residue distance (Å) B Occ A:Hg504 b:0.4 occ:1.00 SG A:CYS300 2.9 0.7 1.0 SG A:CYS66 3.1 0.0 1.0 CA A:CYS66 3.5 0.9 1.0 O A:CYS66 3.6 0.1 1.0 CB A:CYS66 3.8 0.5 1.0 O A:CYS300 3.9 0.6 1.0 CB A:PHE301 3.9 0.4 1.0 C A:CYS300 4.0 0.8 1.0 C A:CYS66 4.0 0.6 1.0 CG1 A:ILE159 4.1 0.6 1.0 N A:PHE301 4.1 0.2 1.0 CA A:PHE301 4.2 0.4 1.0 CB A:CYS300 4.2 0.7 1.0 C A:GLY69 4.4 0.4 1.0 CD1 A:LEU78 4.4 0.0 1.0 CA A:GLY69 4.5 0.7 1.0 CD1 A:PHE301 4.5 0.7 1.0 O A:GLY69 4.6 0.0 1.0 N A:ALA70 4.6 0.9 1.0 N A:CYS66 4.6 0.7 1.0 CD1 A:ILE159 4.7 0.4 1.0 CG A:PHE301 4.7 0.0 1.0 CA A:CYS300 4.7 0.8 1.0 CG A:LEU78 4.9 0.8 1.0 CD2 A:LEU78 5.0 0.2 1.0

Mercury binding site 2 out of 3 in the Crystal Structure of Gltph L66C-S300C Mutant Crosslinked with Divalent Mercury


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 2 of Crystal Structure of Gltph L66C-S300C Mutant Crosslinked with Divalent Mercury within 5.0Å range: probe atom residue distance (Å) B Occ B:Hg504 b:0.9 occ:1.00 SG B:CYS300 3.1 0.9 1.0 CD1 B:ILE159 3.3 0.7 1.0 SG B:CYS66 3.3 0.5 1.0 CG1 B:ILE159 3.5 0.9 1.0 O B:CYS66 3.6 0.6 1.0 CA B:CYS66 3.7 0.9 1.0 CA B:GLY69 4.0 0.9 1.0 C B:GLY69 4.1 0.1 1.0 O B:CYS300 4.1 0.6 1.0 CB B:CYS66 4.1 0.2 1.0 C B:CYS66 4.1 0.5 1.0 C B:CYS300 4.3 0.8 1.0 CB B:PHE301 4.3 0.0 1.0 N B:ALA70 4.4 0.0 1.0 O B:GLY69 4.4 0.0 1.0 N B:PHE301 4.4 0.1 1.0 CB B:CYS300 4.5 0.7 1.0 CA B:PHE301 4.7 0.4 1.0 N B:GLY69 4.8 0.7 1.0 N B:CYS66 4.8 0.5 1.0 CD1 B:LEU78 4.8 0.3 1.0 CB B:ILE159 4.9 0.9 1.0 CA B:CYS300 5.0 0.1 1.0

Mercury binding site 3 out of 3 in the Crystal Structure of Gltph L66C-S300C Mutant Crosslinked with Divalent Mercury


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 3 of Crystal Structure of Gltph L66C-S300C Mutant Crosslinked with Divalent Mercury within 5.0Å range: probe atom residue distance (Å) B Occ C:Hg504 b:0.7 occ:1.00 SG C:CYS66 2.9 0.8 1.0 O C:GLY69 3.4 0.7 1.0 CA C:CYS66 3.6 0.0 1.0 O C:CYS300 3.7 0.5 1.0 SG C:CYS300 3.7 0.6 1.0 O C:CYS66 3.7 0.4 1.0 CB C:CYS66 3.8 0.2 1.0 C C:GLY69 3.8 0.9 1.0 CB C:PHE301 4.0 0.7 1.0 C C:CYS300 4.1 0.9 1.0 C C:CYS66 4.1 1.0 1.0 CA C:GLY69 4.2 0.3 1.0 CG1 C:ILE159 4.2 0.3 1.0 N C:PHE301 4.2 0.2 1.0 CD1 C:LEU78 4.3 0.1 1.0 CA C:PHE301 4.4 1.0 1.0 N C:ALA70 4.6 0.5 1.0 O C:GLY297 4.6 0.3 1.0 N C:CYS66 4.8 1.0 1.0 CB C:CYS300 4.8 0.8 1.0 N C:GLY69 4.8 0.0 1.0 CD1 C:PHE301 4.9 0.6 1.0 CG C:PHE301 4.9 0.1 1.0 CD1 C:ILE159 4.9 0.3 1.0 O C:GLU296 4.9 0.1 1.0

N.Reyes, S.Oh, O.Boudker. Binding Thermodynamics of A Glutamate Transporter Homolog. Nat.Struct.Mol.Biol. V. 20 634 2013.
ISSN: ISSN 1545-9993
PubMed: 23563139
DOI: 10.1038/NSMB.2548