Mercury in PDB 4p6h: Tl+-Bound Inward-Facing State (Bound Conformation) of the Glutamate Transporter Homologue Gltph

The structure of Tl+-Bound Inward-Facing State (Bound Conformation) of the Glutamate Transporter Homologue Gltph, PDB code: 4p6h was solved by G.Verdon, O.Boudker, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	15.00 / 4.08
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	110.830, 200.434, 206.397, 90.00, 90.00, 90.00
R / Rfree (%)	25.8 / 29.6

The structure of Tl+-Bound Inward-Facing State (Bound Conformation) of the Glutamate Transporter Homologue Gltph also contains other interesting chemical elements:

Thallium

(Tl)

6 atoms

The binding sites of Mercury atom in the Tl+-Bound Inward-Facing State (Bound Conformation) of the Glutamate Transporter Homologue Gltph (pdb code 4p6h). This binding sites where shown within 5.0 Angstroms radius around Mercury atom.
In total 3 binding sites of Mercury where determined in the Tl+-Bound Inward-Facing State (Bound Conformation) of the Glutamate Transporter Homologue Gltph, PDB code: 4p6h:
Jump to Mercury binding site number: 1; 2; 3;

Mercury binding site 1 out of 3 in the Tl+-Bound Inward-Facing State (Bound Conformation) of the Glutamate Transporter Homologue Gltph


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 1 of Tl+-Bound Inward-Facing State (Bound Conformation) of the Glutamate Transporter Homologue Gltph within 5.0Å range: probe atom residue distance (Å) B Occ A:Hg501 b:79.7 occ:0.50 SG A:CYS364 1.7 85.1 1.0 CB A:CYS364 2.4 86.8 1.0 SG A:CYS55 3.0 94.9 1.0 CD2 A:LEU374 3.2 89.9 1.0 CB A:CYS55 3.6 90.6 1.0 CA A:CYS55 3.7 89.5 1.0 CA A:CYS364 3.7 87.4 1.0 CD1 A:LEU374 3.8 93.1 1.0 CG A:LEU374 4.1 92.4 1.0 N A:CYS55 4.3 90.2 1.0 N A:CYS364 4.5 86.0 1.0 O A:ILE361 4.6 85.8 1.0 NE2 A:HIS368 4.6 0.4 1.0 C A:CYS364 4.7 89.7 1.0 OH A:TYR383 4.9 86.8 1.0 CD2 A:HIS368 4.9 0.7 1.0 CB A:VAL58 4.9 87.8 1.0 C A:CYS55 4.9 87.0 1.0 CB A:LEU374 5.0 94.3 1.0

Mercury binding site 2 out of 3 in the Tl+-Bound Inward-Facing State (Bound Conformation) of the Glutamate Transporter Homologue Gltph


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 2 of Tl+-Bound Inward-Facing State (Bound Conformation) of the Glutamate Transporter Homologue Gltph within 5.0Å range: probe atom residue distance (Å) B Occ B:Hg501 b:55.3 occ:0.50 SG B:CYS364 1.7 61.4 1.0 CB B:CYS364 2.4 60.1 1.0 SG B:CYS55 3.0 52.0 1.0 CD2 B:LEU374 3.2 67.6 1.0 CB B:CYS55 3.6 49.1 1.0 CA B:CYS55 3.7 48.6 1.0 CA B:CYS364 3.7 60.3 1.0 CD1 B:LEU374 3.8 66.4 1.0 CG B:LEU374 4.1 68.1 1.0 N B:CYS55 4.3 50.3 1.0 N B:CYS364 4.5 60.5 1.0 O B:ILE361 4.6 61.0 1.0 NE2 B:HIS368 4.6 70.8 1.0 C B:CYS364 4.7 60.2 1.0 OH B:TYR383 4.8 69.5 1.0 CD2 B:HIS368 4.9 68.8 1.0 CB B:VAL58 4.9 42.6 1.0 C B:CYS55 5.0 47.8 1.0 CB B:LEU374 5.0 70.4 1.0

Mercury binding site 3 out of 3 in the Tl+-Bound Inward-Facing State (Bound Conformation) of the Glutamate Transporter Homologue Gltph


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 3 of Tl+-Bound Inward-Facing State (Bound Conformation) of the Glutamate Transporter Homologue Gltph within 5.0Å range: probe atom residue distance (Å) B Occ C:Hg501 b:99.3 occ:0.50 SG C:CYS364 1.7 0.1 1.0 CB C:CYS364 2.4 0.4 1.0 SG C:CYS55 3.0 88.2 1.0 CD2 C:LEU374 3.2 0.4 1.0 CB C:CYS55 3.6 82.3 1.0 CA C:CYS55 3.7 80.8 1.0 CA C:CYS364 3.7 0.6 1.0 CD1 C:LEU374 3.8 0.9 1.0 CG C:LEU374 4.1 0.2 1.0 N C:CYS55 4.3 84.0 1.0 N C:CYS364 4.5 0.7 1.0 O C:ILE361 4.6 0.8 1.0 NE2 C:HIS368 4.6 0.4 1.0 C C:CYS364 4.7 0.4 1.0 OH C:TYR383 4.9 0.1 1.0 CD2 C:HIS368 4.9 0.2 1.0 CB C:VAL58 4.9 72.2 1.0 C C:CYS55 5.0 77.5 1.0 CB C:LEU374 5.0 0.8 1.0

G.Verdon, S.Oh, R.N.Serio, O.Boudker. Coupled Ion Binding and Structural Transitions Along the Transport Cycle of Glutamate Transporters. Elife V. 3 02283 2014.
ISSN: ESSN 2050-084X
PubMed: 24842876
DOI: 10.7554/ELIFE.02283