Mercury in PDB 6wzb: Crystal Structure of the Gltph V216C-G388C Mutant Cross-Linked with Divalent Mercury

The structure of Crystal Structure of the Gltph V216C-G388C Mutant Cross-Linked with Divalent Mercury, PDB code: 6wzb was solved by I.Chen, J.Font, R.Ryan, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.31 / 3.45
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	112.111, 204.573, 207.372, 90, 90, 90
R / Rfree (%)	23.3 / 26.8

The structure of Crystal Structure of the Gltph V216C-G388C Mutant Cross-Linked with Divalent Mercury also contains other interesting chemical elements:

Sodium

(Na)

6 atoms

The binding sites of Mercury atom in the Crystal Structure of the Gltph V216C-G388C Mutant Cross-Linked with Divalent Mercury (pdb code 6wzb). This binding sites where shown within 5.0 Angstroms radius around Mercury atom.
In total 3 binding sites of Mercury where determined in the Crystal Structure of the Gltph V216C-G388C Mutant Cross-Linked with Divalent Mercury, PDB code: 6wzb:
Jump to Mercury binding site number: 1; 2; 3;

Mercury binding site 1 out of 3 in the Crystal Structure of the Gltph V216C-G388C Mutant Cross-Linked with Divalent Mercury


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 1 of Crystal Structure of the Gltph V216C-G388C Mutant Cross-Linked with Divalent Mercury within 5.0Å range: probe atom residue distance (Å) B Occ A:Hg504 b:184.1 occ:1.00 SG A:CYS388 2.6 121.1 1.0 CD1 A:ILE213 3.2 107.7 1.0 CB A:CYS216 3.2 130.4 1.0 CB A:CYS388 3.2 115.2 1.0 SG A:CYS216 3.2 152.6 1.0 O A:MET385 3.5 117.0 1.0 CA A:MET385 3.6 95.2 1.0 C A:MET385 4.0 102.9 1.0 CD1 A:LEU228 4.0 100.2 1.0 O A:LEU212 4.0 103.2 1.0 CG A:MET385 4.2 98.9 1.0 CA A:ILE213 4.3 97.9 1.0 O A:ALA384 4.3 103.6 1.0 CB A:MET385 4.3 92.6 1.0 CG1 A:ILE213 4.4 103.9 1.0 C A:LEU212 4.6 96.9 1.0 CG1 A:VAL224 4.6 127.6 1.0 N A:MET385 4.6 94.1 1.0 CB A:ILE213 4.6 98.9 1.0 CA A:CYS388 4.6 111.6 1.0 N A:ILE213 4.7 97.0 1.0 CA A:CYS216 4.7 115.0 1.0 C A:ALA384 4.8 95.8 1.0 CE A:MET385 5.0 110.8 1.0 N A:CYS388 5.0 110.8 1.0

Mercury binding site 2 out of 3 in the Crystal Structure of the Gltph V216C-G388C Mutant Cross-Linked with Divalent Mercury


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 2 of Crystal Structure of the Gltph V216C-G388C Mutant Cross-Linked with Divalent Mercury within 5.0Å range: probe atom residue distance (Å) B Occ B:Hg504 b:185.0 occ:1.00 SG B:CYS388 2.5 106.2 1.0 CB B:CYS216 3.2 122.6 1.0 SG B:CYS216 3.3 129.1 1.0 CB B:CYS388 3.3 100.1 1.0 CD1 B:ILE213 3.4 94.7 1.0 O B:MET385 3.5 118.9 1.0 CA B:MET385 3.5 100.5 1.0 C B:MET385 4.0 108.5 1.0 O B:LEU212 4.0 115.0 1.0 CG B:MET385 4.1 105.8 1.0 O B:ALA384 4.1 106.9 1.0 CB B:MET385 4.3 98.8 1.0 CD1 B:LEU228 4.3 81.8 1.0 CA B:ILE213 4.3 96.9 1.0 N B:MET385 4.5 96.5 1.0 C B:LEU212 4.5 108.7 1.0 CG1 B:ILE213 4.6 93.7 1.0 CA B:CYS388 4.7 97.7 1.0 C B:ALA384 4.7 97.1 1.0 N B:ILE213 4.7 100.7 1.0 CA B:CYS216 4.7 120.7 1.0 CB B:ILE213 4.7 91.0 1.0 CG1 B:VAL224 4.7 115.7 1.0 N B:CYS388 5.0 95.2 1.0 CE B:MET385 5.0 118.6 1.0

Mercury binding site 3 out of 3 in the Crystal Structure of the Gltph V216C-G388C Mutant Cross-Linked with Divalent Mercury


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 3 of Crystal Structure of the Gltph V216C-G388C Mutant Cross-Linked with Divalent Mercury within 5.0Å range: probe atom residue distance (Å) B Occ C:Hg504 b:200.7 occ:1.00 SG C:CYS388 2.5 113.7 1.0 CB C:CYS216 3.2 143.9 1.0 SG C:CYS216 3.2 159.8 1.0 CB C:CYS388 3.2 111.6 1.0 CD1 C:ILE213 3.3 104.5 1.0 O C:MET385 3.6 125.1 1.0 CA C:MET385 3.6 108.5 1.0 O C:LEU212 4.0 127.6 1.0 C C:MET385 4.0 114.1 1.0 CG C:MET385 4.1 103.6 1.0 CD1 C:LEU228 4.2 107.0 1.0 CA C:ILE213 4.2 110.7 1.0 CB C:MET385 4.3 99.2 1.0 O C:ALA384 4.3 113.3 1.0 CG1 C:ILE213 4.5 100.2 1.0 C C:LEU212 4.5 114.3 1.0 N C:MET385 4.6 111.9 1.0 CG1 C:VAL224 4.6 164.3 1.0 CB C:ILE213 4.7 105.2 1.0 CA C:CYS388 4.7 119.1 1.0 CA C:CYS216 4.7 133.9 1.0 N C:ILE213 4.7 112.2 1.0 C C:ALA384 4.8 111.5 1.0 CE C:MET385 5.0 114.9 1.0

I.Chen, S.Pant, Q.Wu, R.Cater, M.Sobti, R.Vandenberg, A.G.Stewart, E.Tajkhorshid, J.Font, R.M.Ryan. Glutamate Transporters Have A Chloride Channel with Two Hydrophobic Gates Nature 2021.
ISSN: ESSN 1476-4687
DOI: 10.1038/S41586-021-03240-9