Mercury in PDB 7ek7: Prawn Ferritin to Coordinate with Heavy Metal Ions

All present enzymatic activity of Prawn Ferritin to Coordinate with Heavy Metal Ions:
1.16.3.1;

The structure of Prawn Ferritin to Coordinate with Heavy Metal Ions, PDB code: 7ek7 was solved by Y.Wang, J.Zang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.02 / 2.70
Space group	I 4
Cell size a, b, c (Å), α, β, γ (°)	126.285, 126.285, 177.247, 90, 90, 90
R / Rfree (%)	25.4 / 33.6

The binding sites of Mercury atom in the Prawn Ferritin to Coordinate with Heavy Metal Ions (pdb code 7ek7). This binding sites where shown within 5.0 Angstroms radius around Mercury atom.
In total 4 binding sites of Mercury where determined in the Prawn Ferritin to Coordinate with Heavy Metal Ions, PDB code: 7ek7:
Jump to Mercury binding site number: 1; 2; 3; 4;

Mercury binding site 1 out of 4 in the Prawn Ferritin to Coordinate with Heavy Metal Ions


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 1 of Prawn Ferritin to Coordinate with Heavy Metal Ions within 5.0Å range: probe atom residue distance (Å) B Occ C:Hg201 b:158.9 occ:1.00 OD1 C:ASN123 2.3 89.9 1.0 SG C:CYS13 2.5 48.1 1.0 CG C:ASN123 2.9 91.6 1.0 CB C:ASN123 2.9 85.8 1.0 O C:THR119 3.1 67.8 1.0 CG2 C:THR119 3.2 60.6 1.0 O C:HOH316 3.6 28.1 1.0 N C:CYS13 3.6 47.4 1.0 C C:THR119 3.7 58.1 1.0 CB C:CYS13 3.8 51.9 1.0 CA C:CYS13 4.0 52.0 1.0 C C:ASP12 4.2 51.0 1.0 CA C:ASN123 4.2 67.3 1.0 ND2 C:ASN123 4.2 70.6 1.0 N C:ASN123 4.3 57.8 1.0 CB C:HIS10 4.3 38.1 1.0 N C:ALA120 4.4 55.1 1.0 CB C:THR119 4.4 65.6 1.0 CA C:THR119 4.5 42.1 1.0 CA C:ALA120 4.5 55.0 1.0 CB C:ASP12 4.6 47.9 1.0 O C:ASP12 4.6 53.0 1.0 O C:HIS10 4.7 28.3 1.0 CA C:ASP12 4.8 58.6 1.0 C C:ASN123 5.0 60.8 1.0

Mercury binding site 2 out of 4 in the Prawn Ferritin to Coordinate with Heavy Metal Ions


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 2 of Prawn Ferritin to Coordinate with Heavy Metal Ions within 5.0Å range: probe atom residue distance (Å) B Occ A:Hg201 b:374.9 occ:1.00 OD1 A:ASN123 2.1 62.1 1.0 SG A:CYS13 2.5 64.9 1.0 CG A:ASN123 2.7 100.5 1.0 N A:CYS13 2.7 74.4 1.0 CB A:ASP12 3.2 95.4 1.0 CB A:HIS10 3.2 76.0 1.0 ND2 A:ASN123 3.2 106.2 1.0 CB A:CYS13 3.4 76.0 1.0 O A:HIS10 3.6 65.7 1.0 C A:ASP12 3.6 72.0 1.0 CA A:CYS13 3.6 61.3 1.0 C A:HIS10 3.6 72.7 1.0 N A:ASP12 3.7 75.1 1.0 CA A:ASP12 3.7 78.4 1.0 O A:HOH310 3.7 46.3 1.0 CB A:ASN123 3.7 88.8 1.0 CA A:HIS10 3.9 73.9 1.0 N A:GLU11 4.2 85.8 1.0 CG A:ASP12 4.2 94.8 1.0 CG A:HIS10 4.4 88.5 1.0 N A:HIS10 4.5 80.4 1.0 C A:GLU11 4.5 65.9 1.0 O A:THR119 4.6 56.3 1.0 ND1 A:HIS10 4.7 78.5 1.0 OD2 A:ASP12 4.7 96.8 1.0 CG2 A:THR119 4.7 53.6 1.0 O A:ASP12 4.7 72.9 1.0 C A:CYS13 4.9 54.8 1.0 CA A:GLU11 5.0 71.6 1.0

Mercury binding site 3 out of 4 in the Prawn Ferritin to Coordinate with Heavy Metal Ions


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 3 of Prawn Ferritin to Coordinate with Heavy Metal Ions within 5.0Å range: probe atom residue distance (Å) B Occ D:Hg201 b:91.4 occ:1.00 SG D:CYS13 2.6 85.3 1.0 OD1 D:ASN123 2.8 79.1 1.0 CG2 D:THR119 3.0 45.5 1.0 O D:THR119 3.3 58.2 1.0 CG D:ASN123 3.5 72.0 1.0 N D:CYS13 3.7 60.2 1.0 C D:THR119 3.7 56.2 1.0 CB D:ASN123 3.8 65.4 1.0 CB D:HIS10 3.9 73.9 1.0 ND1 D:HIS10 3.9 76.9 1.0 CB D:ASP12 3.9 64.3 1.0 CB D:CYS13 4.0 69.7 1.0 CB D:THR119 4.1 47.8 1.0 O D:HIS10 4.2 62.5 1.0 N D:ALA120 4.3 63.1 1.0 CA D:CYS13 4.3 56.4 1.0 CA D:ALA120 4.3 80.2 1.0 CG D:HIS10 4.4 79.2 1.0 CA D:THR119 4.4 52.4 1.0 C D:ASP12 4.5 64.4 1.0 ND2 D:ASN123 4.6 74.2 1.0 CA D:ASP12 4.7 57.1 1.0 C D:HIS10 4.9 63.2 1.0 CA D:HIS10 4.9 58.6 1.0 N D:ASP12 4.9 48.4 1.0 O D:ALA120 4.9 70.2 1.0 N D:ASN123 5.0 73.4 1.0

Mercury binding site 4 out of 4 in the Prawn Ferritin to Coordinate with Heavy Metal Ions


Mono view


Stereo pair view

A full contact list of Mercury with other atoms in the Hg binding site number 4 of Prawn Ferritin to Coordinate with Heavy Metal Ions within 5.0Å range: probe atom residue distance (Å) B Occ E:Hg201 b:182.9 occ:1.00 SG E:CYS13 2.5 91.1 1.0 ND2 E:ASN123 2.5 92.4 1.0 CB E:HIS10 3.3 55.9 1.0 CG E:ASN123 3.4 68.5 1.0 CB E:ASN123 3.5 53.5 1.0 CG2 E:THR119 3.6 57.4 1.0 O E:HOH308 3.6 41.5 1.0 CB E:CYS13 3.6 68.6 1.0 N E:CYS13 3.8 64.0 1.0 O E:THR119 3.9 54.0 1.0 CG E:HIS10 4.1 64.2 1.0 CA E:CYS13 4.2 53.5 1.0 C E:THR119 4.3 56.7 1.0 CA E:HIS10 4.3 57.6 1.0 CA E:ALA120 4.4 42.5 1.0 N E:HIS10 4.5 55.5 1.0 ND1 E:HIS10 4.5 66.7 1.0 OD1 E:ASN123 4.6 70.6 1.0 N E:ALA120 4.7 46.8 1.0 C E:HIS10 4.7 51.8 1.0 C E:ASP12 4.7 58.4 1.0 O E:HIS10 4.7 45.1 1.0 CA E:ASN123 4.7 56.4 1.0 N E:ASN123 4.8 63.2 1.0 CB E:THR119 4.9 63.3 1.0 N E:ASP12 5.0 40.9 1.0

Y.Wang, J.Zang, C.Wang, X.Zhang, G.Zhao. Structural Insights For the Stronger Ability of Shrimp Ferritin to Coordinate with Heavy Metal Ions As Compared to Human H-Chain Ferritin. Int J Mol Sci V. 22 2021.
ISSN: ESSN 1422-0067
PubMed: 34360624
DOI: 10.3390/IJMS22157859